Fluorescent immunochromatography of vancomycin in plasma: A rapid and sensitive quantitative analysis.

Vancomycin (VAN) has a narrow therapeutic window and variable pharmacokinetic properties, which require timely monitoring of plasma concentration to adjust the dosage for better effectiveness. A sensitive and quantitative immunochromatographic method was developed to detect VAN in plasma. The linear response range of the method to detect plasma VAN was 1.25-50 μg/mL. The intra- and inter-assay coefficients were 4.68% and 8.81%, respectively, while the recovery was 89.10%-98.32%. The clinical comparative experiment results demonstrated a good correlation (r > 0.90) between the fluorescent immunochromatographic strip test and the mass spectrum. In this study, a simple, rapid, and high-sensitivity immunochromatographic method was established for detecting VAN, which helped establish the basis for further application of monitoring plasma concentration. © 2023 John Wiley & Sons Ltd.

Citation

Minmin Shen, Haiying Gu, Kai Deng, Zhongpeng Li, Yumei Yuan, Yangjin He, Yin Jin, Nengming Lin. Fluorescent immunochromatography of vancomycin in plasma: A rapid and sensitive quantitative analysis. Biomedical chromatography : BMC. 2023 Aug;37(8):e5656

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PMID: 37060570

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