Yonggang Zhao, Yang Xiong, Jingchuan Zheng, Wenyao Kongling, Junlin Chen, Chengli Li, Peilun Hu, Shuhui Yang, Xiumei Wang
Biomaterials science 2023 May 30An artificial periosteum has emerged as an encouraging candidate for bone defect repair. Currently, it remains a great challenge to develop a multifaceted biomimetic periosteum integrating multifunctional features of bioactivities and unique mechanical properties. Here, we successfully fabricated an artificial periosteum (AP) composed of hierarchically assembled Mg-doped mineralized collagen microfibrils with a biomimetically rotated lamellar structure via a "multiscale cascade regulation" strategy combining multiple techniques such as molecular self-assembly, electrospinning, and pressure-driven fusion from molecular to macroscopic levels. The AP has excellent mechanical properties with an ultimate strength and a tensile modulus of 15.9 MPa and 1.1 GPa, respectively. The involvement of Mg-doped nano-hydroxyapatite endowed the AP with good osteogenic and angiogenic activities to promote osteogenic differentiation of bone marrow mesenchymal stem cells and human umbilical vein endothelial cell differentiation into capillary-like structures in vitro. In addition, the results of in vivo evaluations in a rat cranial bone defect model including micro-CT morphology, histological staining, and immunohistochemical analysis showed that Mg-doped mineralized collagen-based AP (MgMC@AP) significantly facilitated cranial bone regeneration and fast vascularization. Our findings suggest that the AP mimicked the composition, lamellar structure, mechanical properties, and biological activities of natural periosteum/lamellae, showing great promise for bone tissue regeneration.
Yonggang Zhao, Yang Xiong, Jingchuan Zheng, Wenyao Kongling, Junlin Chen, Chengli Li, Peilun Hu, Shuhui Yang, Xiumei Wang. A multifaceted biomimetic periosteum with a lamellar architecture and osteogenic/angiogenic dual bioactivities. Biomaterials science. 2023 May 30;11(11):3878-3892
PMID: 37078909
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