Production of Enhanced Carotenoid-Producing Strains of the Yeast Rhodotorula gracilis Using the Antibiotic Zeocin.

Yusuke Watabe, Shouji Takahashi

Applied biochemistry and biotechnology 2023 Dec

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We employed genomic mutagenesis to the yeast Rhodotorula gracilis using Zeocin as a mutagen to develop enhanced carotenoid-producing mutant strains. The yeast mutant strains with enhanced carotenoid pigmentation were produced on agar plates containing Zeocin at a concentration of less than 1.5 µg/mL. The optimum concentration for producing enhanced carotenoid-pigmentation mutant strains was 0.25 µg/mL. The production of β-carotene, torulene, and torularhodin in an enhanced pigmentation strain were 1.3, 1.65, and 1.5 times higher than in the wild-type strain. These results suggested that genomic mutagenesis of the yeast using Zeocin could be applied for efficiently producing enhanced carotenoid-producing mutant strains. © 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Yusuke Watabe, Shouji Takahashi. Production of Enhanced Carotenoid-Producing Strains of the Yeast Rhodotorula gracilis Using the Antibiotic Zeocin. Applied biochemistry and biotechnology. 2023 Dec;195(12):7889-7897