Preparation and Analysis of Pueraria lobata Polysaccharides.

Pueraria lobata polysaccharides (PLPs) were obtained by a hot water extraction method. Starting from the single factor experiment, the extraction was optimized by response surface methodology, and the following optimal extraction parameters were obtained: the extraction temperature was 84 °C, the liquid-solid ratio was 11 mL/g, the extraction time was 73 min, and the extraction rate of polysaccharides was 8.59%. The Sevag method was used to remove the protein soluble in water and H2O2 was used to remove the pigment; then PLPs were precipitated with three times of anhydrous ethanol, soluble salts and other small molecules were removed by dialysis, and finally refined PLPs were obtained by freeze-drying.

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Yumin Fan, Gangliang Huang. Preparation and Analysis of Pueraria lobata Polysaccharides. ACS biomaterials science & engineering. 2023 May 08;9(5):2329-2334

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PMID: 37104693

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