Protective effects of resolvin D1 in Pseudomonas aeruginosa keratitis.

Jiayin Wu, Jianlu Gao, Lili Yi, Nan Gao, Leyi Wang, Jing Zhu, Chenyang Dai, Lin Sun, Hui Guo, Fu-Shin X Yu, Xinyi Wu

Molecular immunology 2023 Jun

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Here, we explored the protective effects of resolvin D1 (RvD1) in Pseudomonas aeruginosa (PA) keratitis. C57BL/6 (B6) mice were used as an animal model of PA keratitis. Plate counting and clinical scores were used to assess the severity of the infection and the therapeutic effects of RvD1 in the model. Myeloperoxidase assay was used to detect neutrophil infiltration and activity. Quantitative PCR (qPCR) was used to examine the expression of proﬂammatory and anti-inﬂammatory mediators. Immunoﬂuorescence staining and qPCR were performed to identify macrophage polarization. RvD1 treatment alleviated PA keratitis severity by decreasing corneal bacterial load and inhibiting neutrophil infiltration in the mouse model. Furthermore, RvD1 treatment decreased mRNA levels of TNF-α, IFN-γ, IL-1β, CXCL1, and S100A8/9 while increasing those of IL-1RA, IL-10, and TGF-β1. RvD1 treatment also reduced the aggregation of M1 macrophages and increased that of M2 macrophages. RvD1 provided an auxiliary effect in gatifloxacin-treated mice with PA keratitis. Based on these findings, RvD1 may improve the prognosis of PA keratitis by inhibiting neutrophil recruitment and activity, dampening the inﬂammatory response, and promoting M2 macrophage polarization. Thus, RvD1 may be a potential complementary therapy for PA keratitis. Copyright © 2023. Published by Elsevier Ltd.

Citation

Jiayin Wu, Jianlu Gao, Lili Yi, Nan Gao, Leyi Wang, Jing Zhu, Chenyang Dai, Lin Sun, Hui Guo, Fu-Shin X Yu, Xinyi Wu. Protective effects of resolvin D1 in Pseudomonas aeruginosa keratitis. Molecular immunology. 2023 Jun;158:35-42