NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b.

Bruno Hargittay, Konstantin S Mineev, Christian Richter, Sridhar Sreeramulu, Hendrik R A Jonker, Krishna Saxena, Harald Schwalbe

Biomolecular NMR assignments 2023 Jun

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The splicing isoform b of human fibroblast growth factor 8 (FGF8b) is an important regulator of brain embryonic development. Here, we report the almost complete NMR chemical shift assignment of the backbone and aliphatic side chains of FGF8b. Obtained chemical shifts are in good agreement with the previously reported X-ray data, excluding the N-terminal gN helix, which apparently forms only in complex with the receptor. The reported data provide an NMR starting point for the investigation of FGF8b interaction with its receptors and with potential drugs or inhibitors. © 2023. The Author(s).

Citation

Bruno Hargittay, Konstantin S Mineev, Christian Richter, Sridhar Sreeramulu, Hendrik R A Jonker, Krishna Saxena, Harald Schwalbe. NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b. Biomolecular NMR assignments. 2023 Jun;17(1):135-142