Dynamic changes of the Prf/Pto tomato resistance complex following effector recognition.

In both plants and animals, nucleotide-binding leucine-rich repeat (NLR) immune receptors play critical roles in pathogen recognition and activation of innate immunity. In plants, NLRs recognise pathogen-derived effector proteins and initiate effector-triggered immunity (ETI). However, the molecular mechanisms that link NLR-mediated effector recognition and downstream signalling are not fully understood. By exploiting the well-characterised tomato Prf/Pto NLR resistance complex, we identified the 14-3-3 proteins TFT1 and TFT3 as interacting partners of both the NLR complex and the protein kinase MAPKKKα. Moreover, we identified the helper NRC proteins (NLR-required for cell death) as integral components of the Prf /Pto NLR recognition complex. Notably our studies revealed that TFTs and NRCs interact with distinct modules of the NLR complex and, following effector recognition, dissociate facilitating downstream signalling. Thus, our data provide a mechanistic link between activation of immune receptors and initiation of downstream signalling cascades. © 2023. The Author(s).

Citation

Arsheed H Sheikh, Iosif Zacharia, Alonso J Pardal, Ana Dominguez-Ferreras, Daniela J Sueldo, Jung-Gun Kim, Alexi Balmuth, Jose R Gutierrez, Brendon F Conlan, Najeeb Ullah, Olivia M Nippe, Anil M Girija, Chih-Hang Wu, Guido Sessa, Alexandra M E Jones, Murray R Grant, Miriam L Gifford, Mary Beth Mudgett, John P Rathjen, Vardis Ntoukakis. Dynamic changes of the Prf/Pto tomato resistance complex following effector recognition. Nature communications. 2023 May 04;14(1):2568

Mesh Tags

Substances

PMID: 37142566

search → result