Enhanced effect of secondary administrations of adipose-derived stromal cells concurrent with fat grafting.

Cell-assisted lipotransfer, a fat graft mixed with adipose-derived stromal cells, is known to enhance fat graft retention. Previously, we showed that intravenous injection of adipose-derived stromal cells can improve the survival of grafted fat. In the present study, we investigated the effects of a secondary intravenous injection of adipose-derived stromal cells on fat grafting. Wild-type C57BL/6J (B6) mice were used as donors for grafted fat and as recipients. Adipose-derived stromal cells were harvested from green fluorescent protein and DsRed B6 mice. The recipient mice were divided into three groups: SI (n = 10), RI1 (n = 10), and RI2 (n = 11). All groups received intravenous injections of green fluorescent protein adipose-derived stromal cells immediately after fat grafting. The RI1 and RI2 groups received repeated intravenous injections of DsRed adipose-derived stromal cells at 1 and 2 weeks, respectively, after fat grafting. The grafted fat volume was measured using micro-computed tomography. Secondarily injected DsRed adipose-derived stromal cells were recruited to the grafted fat and resulted in a higher retention of graft volume and vascular density (p < 0.05). The stromal-derived factor-1 and C-X-C chemokine receptor type 4 genes related to stem cell homing were highly expressed in the grafted fat and adipose-derived stromal cells (p < 0.05). The RI2 group showed a higher graft volume and vascular density than the SI and RI1 groups (p < 0.05). A secondary intravenous injection of adipose-derived stromal cells at a 2-week interval enhances the effect of adipose-derived stromal cell enrichment in fat grafting. These findings refine clinical protocols and enhance the therapeutic value of cell-assisted lipotransfer. Copyright © 2023 by the American Society of Plastic Surgeons.

Citation

Yan Huang, Xian Jin, Ki Yong Hong, Hak Chang. Enhanced effect of secondary administrations of adipose-derived stromal cells concurrent with fat grafting. Plastic and reconstructive surgery. 2023 May 09

PMID: 37159916

search → result