Modulating liposomal nanoparticles to enhance uptake and targeting of methicillin-resistant Staphylococcus aureus.

Aims: To explore the role of modifying the phospholipid composition of liposomal nanoparticles (LNPs) on their uptake. Methods: Different LNPs were labeled with a fluorescent marker and their uptake by human lung fibroblast (WI-38) cells was evaluated using flow cytometry and confocal microscopy. Linezolid was loaded in LNPs showing enhanced uptake, and their ability to reduce intracellular methicillin-resistant Staphylococcus aureus (MRSA) was investigated by in vitro infection. Results: Liposomes with disaturated dipalmitoylphosphatidylcholine-phosphatidylglycerol-phosphatidylethanolamine at a molar ratio of 60:10:10, mimicking that of WI-38 cells, were more effectively uptaken. Linezolid-loaded LNPs significantly reduced intracellular MRSA viable count. Conclusion: Modified LNPs could be promising antibiotic nanocarriers for targeting intracellular MRSA, which are usually resistant to conventional antibiotics.

Citation

Mona Elhabak, Rania Ibrahim Shebl, Samia Omar. Modulating liposomal nanoparticles to enhance uptake and targeting of methicillin-resistant Staphylococcus aureus. Future microbiology. 2023 Apr;18:343-355

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PMID: 37166177

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