Visualizing Metal Distribution in Plants Using Synchrotron X-Ray Fluorescence Microscopy Techniques.

Ju-Chen Chia, Arthur R Woll, Louisa Smieska, Olena K Vatamaniuk

Methods in molecular biology (Clifton, N.J.) 2023

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Recent improvements in synchrotron-based X-ray fluorescence (SXRF) microscopy established it as an advanced analytical tool for analyzing 2D- and 3D distribution of mineral elements in plants. Among existing imaging techniques, SXRF microscopy offers several unique capabilities, including in situ metal quantification in plant tissues and high sensitivity, as low as 1 mg kg-1, at the nanoscale spatial resolution. SXRF is increasingly utilized in different plant science disciplines to provide a fundamental understanding of metal homeostasis, and the function of trace elements in plant metabolism and development. Here, we describe methods for SXRF imaging, including sample preparation, the optimization of conventional SXRF for analyzing trace elements, and the development of confocal SXRF (C-SXRF). © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Ju-Chen Chia, Arthur R Woll, Louisa Smieska, Olena K Vatamaniuk. Visualizing Metal Distribution in Plants Using Synchrotron X-Ray Fluorescence Microscopy Techniques. Methods in molecular biology (Clifton, N.J.). 2023;2665:177-189