Analysis of RNA-Protein Interaction Networks Using RNP-MaP.

Kaushik Saha, Gourisankar Ghosh

Methods in molecular biology (Clifton, N.J.) 2023

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RNA-protein interactions regulate a myriad of biological functions through formation of ribonucleoprotein complexes. These complexes may consist of one or more RNA-protein interaction network(s) providing additional layers of regulatory potential to the RNA. Moreover, since the protein-binding also regulates local and global structure of the RNA by structurally remodeling the latter, it is important to correlate RNA nucleotide flexibility with the site of protein-binding. We have discussed methods for chemical probing of structure of the RNA in the protein-free and protein-bound states in the preceding chapters. In this chapter, we describe a ribonucleoprotein mutational profiling (RNP-MaP) method for probing RNA-protein interaction networks. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Kaushik Saha, Gourisankar Ghosh. Analysis of RNA-Protein Interaction Networks Using RNP-MaP. Methods in molecular biology (Clifton, N.J.). 2023;2666:95-105