Study on the Promotion of hADSCs Migration and Chemotaxis by SDF-1.

The purpose of this study was to investigate the chemotaxis effect of stromal cell-derived factor-1 (SDF-1) on human adipose-derived stem cells (hADSCs). A lentivirus vector with the enhanced green fluorescent protein gene was constructed and transfected to hADSCs. A control group and an SDF-1 induction group were set to estimate the efficacy of SDF-1 in promoting hADSCs chemotaxis and migration. After 7 days of infection with hADSCs by enhanced green fluorescent protein lentivirus, the positive rate of fluorescence expression detected by flow cytometry was 100%. After the addition of SDF-1 induction, the invasion ability of hADSCs was enhanced. SDF-1 can promote hADSCs migration and chemotaxis, which may play a role in stem cell transplantation. Copyright © 2023 Asia-Pacific Academy of Ophthalmology. Published by Wolters Kluwer Health, Inc. on behalf of the Asia-Pacific Academy of Ophthalmology.

Citation

Chen Jin, Guoxing Xu. Study on the Promotion of hADSCs Migration and Chemotaxis by SDF-1. Asia-Pacific journal of ophthalmology (Philadelphia, Pa.). 2023 May-Jun 01;12(3):303-309

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PMID: 37171133

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