Human Autoantigen Atlas: Searching for the Hallmarks of Autoantigens.

Journal of proteome research 2023 Jun 02

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Understanding autoimmunity to endogenous proteins is crucial in diagnosing and treating autoimmune diseases. In this work, we developed a user-friendly AAgAtlas portal (http://biokb.ncpsb.org.cn/aagatlas_portal/index.php#), which can be used to search for 8045 non-redundant autoantigens (AAgs) and 47 post-translationally modified AAgs against 1073 human diseases that are prioritized by a credential score developed by multisource evidence. Using AAgAtlas, the immunogenic properties of human AAgs was systematically elucidated according to their genetic, biophysical, cytological, expression profile, and evolutionary characteristics. The results indicated that human AAgs are evolutionally conserved in protein sequence and enriched in three hydrophilic and polar amino acid residues (K, D, and E) that are located at the protein surface. AAgs are enriched in proteins that are involved in nucleic acid binding, transferase, and the cytoskeleton. Genome, transcriptome, and proteome analyses further indicated that AAb production is associated with gene variance and abnormal protein expression related to the pathological activities of different tumors. Collectively, our data outlines the hallmarks of human AAgs that facilitate the understanding of humoral autoimmunity and the identification of biomarkers of human diseases.

Citation

Dan Wang, Dong Yang, Liuhui Yang, Lihong Diao, Yuqi Zhang, Yang Li, Hongye Wang, Jing Ren, Linlin Cheng, Qiaoyun Tan, Ran Zhang, Xiaohong Han, Xiaohan Zhang, Bingwei Wang, Dong Li, Meng Chen, Henning Hermjakob, Yongzhe Li, Joshua LaBaer, Zhou Zhou, Xiaobo Yu. Human Autoantigen Atlas: Searching for the Hallmarks of Autoantigens. Journal of proteome research. 2023 Jun 02;22(6):1800-1815