Measuring Immune Cell Movement Toward the Soluble Microenvironment of Human Tissues Using a Boyden Chamber-Based Migration Assay.

Eimear Mylod, Joanne Lysaght, Melissa J Conroy

Methods in molecular biology (Clifton, N.J.) 2023

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Migration assays are used to measure cell movement toward a variety of chemoattractants in a controlled environment. Here we describe a method for a Boyden chamber-based migration assay using conditioned media generated from the tumor, liver, and visceral adipose tissue of cancer patients. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Eimear Mylod, Joanne Lysaght, Melissa J Conroy. Measuring Immune Cell Movement Toward the Soluble Microenvironment of Human Tissues Using a Boyden Chamber-Based Migration Assay. Methods in molecular biology (Clifton, N.J.). 2023;2645:231-240