Testing for Factor V Leiden (FVL) and Prothrombin G20210A Genetic Variants.

Laboratory testing for Factor V Leiden and Prothrombin G20210A genetic variants permits defining the increased relative risk for venous thromboembolism in selected patients. Laboratory DNA testing for these variants may be undertaken by a variety of methods, including fluorescence-based quantitative real-time PCR (qPCR). This is a rapid, simple, robust, and reliable method to identify genotypes of interest. This chapter describes the method that employs PCR amplification of the patient DNA region of interest and genotyping by allele-specific discrimination technology on a quantitative real-time PCR (qPCR) instrument. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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Tadej Pajič. Testing for Factor V Leiden (FVL) and Prothrombin G20210A Genetic Variants. Methods in molecular biology (Clifton, N.J.). 2023;2663:233-251

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PMID: 37204714

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