Long-term imaging of intranuclear Mg2+ dynamics during mitosis using a localized fluorescent probe.

A novel fluorescent Mg2+ probe was developed based on a small molecule-protein hybrid. This probe enables subcellular targeting, long-term imaging, and high selectivity for Mg2+ over Ca2+. Using ratiometric fluorescence microscopy with a co-localized standard fluorophore, the variations in intranuclear Mg2+ concentrations during mitosis could be visualized.

Citation

Yusuke Matsui, Toshiyuki Kowada, Yi Ding, Priya Ranjan Sahoo, Kazuya Kikuchi, Shin Mizukami. Long-term imaging of intranuclear Mg2+ dynamics during mitosis using a localized fluorescent probe. Chemical communications (Cambridge, England). 2023 Jun 06;59(46):7048-7051

Mesh Tags

Substances

PMID: 37211865

search → result