Differentiation of hPSCs to Study PRC2 Role in Cell-Fate Specification and Neurodevelopment.

Several studies highlighted the importance of the polycomb repressive complex 2 (PRC2) already at the beginning of development. Although the crucial function of PRC2 in regulating lineage commitment and cell-fate specification has been well-established, the in vitro study of the exact mechanisms for which H3K27me3 is indispensable for proper differentiation is still challenging. In this chapter, we report a well-established and reproducible differentiation protocol to generate striatal medium spiny neurons as a tool to explore PRC2 role in brain development. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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Silvia Brocchetti, Paola Conforti. Differentiation of hPSCs to Study PRC2 Role in Cell-Fate Specification and Neurodevelopment. Methods in molecular biology (Clifton, N.J.). 2023;2655:211-220

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PMID: 37212999

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