Orofacial anti-hypernociceptive effect of citral in acute and persistent inflammatory models in rats.

Bruna M Santos, Wanderson S Santos, Isabelly G Solon, Felipe S Garcia, Maycon T Emilio-Silva, Aline A Jesus, Clélia A Hiruma-Lima, Glauce C Nascimento, Evelin C Cárnio, Luiz G S Branco

Archives of oral biology 2023 Aug

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Orofacial pain has significant psychological and physiological effects. Citral (3,7-dimethyl-2,6-octadienal) is the main component of Cymbopogon citratus (DC) Stapf, an herb with analgesic properties. Although citral has been considered a potent analgesic, its putative effects on orofacial pain are still unknown. The objective of this study is to test the hypothesis that citral modulates orofacial pain using two experimental models: formalin-induced hyperalgesia in the vibrissae area and during persistent temporomandibular hypernociception using Complete Freund's Adjuvant - CFA test. For the formalin test, citral (100 and 300 mg/kg, oral gavage) or its vehicle (Tween 80, 1 %) were given 1 h before the formalin injection subcutaneously (sc) into the vibrissae area. For the CFA model, we analyzed the prophylactic (100 mg/kg of citral by oral gavage, 1 h before CFA injection) and the chronic therapeutic (citral treatment 1-hour post-CFA injection and daily post-CFA injection) effect of citral or its vehicle in animals treated with CFA for 8 days. Citral caused a decrease in formalin-induced local inflammation and the time spent performing nociceptive behavior in a dose-dependent fashion. Similarly, prophylactic and therapeutic citral treatment decreased the CFA-induced persistent mechanical hypernociception in the temporomandibular area. Our data strengthen the notion that citral plays a powerful antinociceptive role by decreasing orofacial hypernociception in formalin and CFA models. Copyright © 2023 Elsevier Ltd. All rights reserved.

Citation

Bruna M Santos, Wanderson S Santos, Isabelly G Solon, Felipe S Garcia, Maycon T Emilio-Silva, Aline A Jesus, Clélia A Hiruma-Lima, Glauce C Nascimento, Evelin C Cárnio, Luiz G S Branco. Orofacial anti-hypernociceptive effect of citral in acute and persistent inflammatory models in rats. Archives of oral biology. 2023 Aug;152:105734