Zehua Huang, Huimin Wu, Junjie Fan, Qixiang Mei, Yang Fu, Nuoming Yin, Binqiang Xu, Shengzheng Luo, Baiwen Li, Jianbo Ni, Chunlan Huang, Junjie Hu, Yue Zeng
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 JulMucin-2 (MUC2) secreted by goblet cells participates in the intestinal barrier, but its mechanism in acute necrotizing pancreatitis (ANP) remains unclear. In acute pancreatitis (AP) patients, the functions of goblet cells (MUC2, FCGBP, CLCA1, and TFF3) decreased, and MUC2 was negatively correlated with AP severity. ANP rats treated with pilocarpine (PILO) (PILO+ANP rats) to deplete MUC2 showed more serious pancreatic and colonic injuries, goblet cell dysfunction, gut dysbiosis, and bacterial translocation than those of ANP rats. GC-MS analysis of feces showed that PILO+ANP rats had lower levels of butyric acid, isobutyric acid, isovaleric acid, and hexanoic acid than those of ANP rats. The expression of MUC2 was associated with colonic injury and gut dysbiosis. All these phenomena could be relieved, and goblet cell functions were also partially reversed by MUC2 supplementation in ANP rats. TNF-α-treated colonoids had exacerbated goblet cell dysfunction. MUC2 expression was negatively correlated with the levels of pro-inflammatory cytokines (IL-1β and IL-6) (p < .05) and positively related to the expression of tight junction proteins (Claudin 1, Occludin, and ZO1) (p < .05). Downregulating MUC2 by siRNA increased the levels of the pro-inflammatory cytokines in colonoids. MUC2 might maintain intestinal homeostasis to alleviate ANP. © 2023 Federation of American Societies for Experimental Biology.
Zehua Huang, Huimin Wu, Junjie Fan, Qixiang Mei, Yang Fu, Nuoming Yin, Binqiang Xu, Shengzheng Luo, Baiwen Li, Jianbo Ni, Chunlan Huang, Junjie Hu, Yue Zeng. Colonic mucin-2 attenuates acute necrotizing pancreatitis in rats by modulating intestinal homeostasis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2023 Jul;37(7):e22994
PMID: 37249555
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