Seasonal changes in the scrotal skin histology in relation to thermoregulation and testosterone level in camel and buffalo bull.

Shakil Ahmed, Anas Sarwar Qureshi, Muhammad Usman, Sarmad Rehan, Farrah Deeba, Muhammad Khalil Ateeq, Muhammad Younus, Muhammad Ahsan Naeem, Zaima Umar, Muhammad Sulman Ali Taseer

Anatomia, histologia, embryologia 2023 Sep

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This study was conducted to compare the scrotal skin dynamics of camel and buffalo bulls regarding thermoregulation, season and testosterone. Scrotal skin and blood samples (n = 8 each) were collected during breeding (October to March) and non-breeding seasons (April to August). Tissue slides were prepared by the paraffin embedding technique followed by hematoxylin and eosin (H&E) staining and were analysed by ImageJ®. Relative humidity and temperature were recorded in different seasons to calculate the thermal humidity index (THI). Serum testosterone level was estimated through radioimmunoassay (RIA). The data were analysed by applying ANOVA, the THS test was used as a post-ANOVA interface and the correlation coefficient was also calculated among season, testosterone and skin parameters. The results showed that skin thickness, season and THI were negatively correlated with testosterone levels. In the neck, body and apex, the papillary layer was thicker in buffalo bull compared to camel bull during the breeding season; however, the reticular layer followed a reverse trend in the apex when THI was low. In the scrotal neck during the non-breeding season, hair follicle (HF) length and density were recorded high in camel bull compared to buffalo bull when THI was elevated. The density of HF followed the otherwise trend in the apex during the breeding season. The density of SG was found highest in all regions of camel bull as compared to buffalo bull and showed direct relation with THI. To conclude, this study delineates how the histo-dynamics of scrotal skin varies and accommodate itself with testosterone and THI over the different season in camel and buffalo. © 2023 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd.

Citation

Shakil Ahmed, Anas Sarwar Qureshi, Muhammad Usman, Sarmad Rehan, Farrah Deeba, Muhammad Khalil Ateeq, Muhammad Younus, Muhammad Ahsan Naeem, Zaima Umar, Muhammad Sulman Ali Taseer. Seasonal changes in the scrotal skin histology in relation to thermoregulation and testosterone level in camel and buffalo bull. Anatomia, histologia, embryologia. 2023 Sep;52(5):752-761