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Hypoxia and its signalling pathway play a key role in human physiology and a variety of diseases. Alterations in histone methylation coordinate transcriptional responses to hypoxia. Here, we detail a fixed cell immunofluorescence method for quantifying hypoxia-induced changes in histone methylation, exemplified by the measurement of H3K27me3. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.


Dilem Shakir, Michael Batie, Sonia Rocha. Fixed Cell Immunofluorescence for Quantification of Hypoxia-Induced Changes in Histone Methylation. Methods in molecular biology (Clifton, N.J.). 2023;2675:261-269

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PMID: 37258769

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