Multifaceted Chiral Probe 2,3-Dihydro-4-hydroxy-chromene Schiff Base in Detecting Cu2+ Ions, l-Histidine, and Indazole: Spectroscopic Investigation and Confocal and Live Cell Imaging.

Mohanasundaram Ranjani, Vasanthakumar Keerthana, Subramaniam Selvakumar, Vincent M Lynch, Amirthalingam Mohankumar, Sundararaj Palanisamy, Palaniappan Kalaivani, Rathinasabapathi Prabhakaran

ACS applied bio materials 2023 Jun 19

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2,3-Dihydro-4-hydroxy-chromene-4(N)-ethyl thiosemicarbazone (probe F4) is used as a chemosensor, and it selectively detected Cu2+ ions among the metal ions by showing fluorescence "TURN ON" behavior. The stoichiometric binding of the probe with Cu2+ (CF4) was confirmed by Job's plot and mass spectroscopy. Further, CF4 was used as a sensor for the detection of l-amino acids and N-heterocyclic compounds. Among them, CF4 selectively detected l-histidine by showing fluorescence "TURN-OFF" behavior and selectively detected indazole by showing fluorescence "TURN-ON" behavior. These behaviors were further confirmed by in vivo live cell imaging studies by using Caenorhabditis elegans as a model. In vitro cytotoxicity was assayed for probe F4, complex CF4, and CF4 with l-histidine and indazole. The IC50 concentration was used for confocal imaging studies by using the MDA-MB-231 cell line (breast cancer cell line).

Citation

Mohanasundaram Ranjani, Vasanthakumar Keerthana, Subramaniam Selvakumar, Vincent M Lynch, Amirthalingam Mohankumar, Sundararaj Palanisamy, Palaniappan Kalaivani, Rathinasabapathi Prabhakaran. Multifaceted Chiral Probe 2,3-Dihydro-4-hydroxy-chromene Schiff Base in Detecting Cu2+ Ions, l-Histidine, and Indazole: Spectroscopic Investigation and Confocal and Live Cell Imaging. ACS applied bio materials. 2023 Jun 19;6(6):2358-2369