Detoxification of p-nitrophenol (PNP) using Enterococcus gallinarum JT-02 isolated from animal farm waste sludge.

Julieta Celeste Martín Tempestti, Harshavardhan Mohan, Pavithra Muthukumar Sathya, Se-Won Lee, Janaki Venkatachalam, Byung-Taek Oh, Kamala-Kannan Seralathan

Environmental research 2023 Aug 15

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Enterococcus gallinarum (JT-02) isolated and identified from the animal farm waste sludge was found to be capable of biodegrading p-nitrophenol (PNP), an organic compound used to manufacture drugs, fungicides, insecticides, dyes, and to darken leather. The intention of this study was to optimize the biodegradation by finding the optimal conditions for the specific strain through single-factor experiments. The bacterial strain was grown in Luria Bertani broth and various parameters were optimized to achieve the prime settings for the p-nitrophenol (PNP) biodegradation. The results indicated that the best setups for the biodegradation by the strain JT-02 was 100 mg/L of PNP; pH 7; 30 °C; 150 rpm in a shaker incubator and 3% (v/v) of inoculum dose. Once the optimal conditions were found, the bacteria were capable of degrading p-nitrophenol (98.21%) in 4 days. Intermediates produced during PNP biodegradation were identified using High Performance Liquid Chromatography (HPLC) analysis and the biodegradation pathway was elucidated. Phytotoxicity studies were carried out with Vigna radiata seeds to confirm the applicability and efficiency of PNP biodegradation. Copyright © 2023 Elsevier Inc. All rights reserved.

Citation

Julieta Celeste Martín Tempestti, Harshavardhan Mohan, Pavithra Muthukumar Sathya, Se-Won Lee, Janaki Venkatachalam, Byung-Taek Oh, Kamala-Kannan Seralathan. Detoxification of p-nitrophenol (PNP) using Enterococcus gallinarum JT-02 isolated from animal farm waste sludge. Environmental research. 2023 Aug 15;231(Pt 3):116289