Effective monitoring of Platinum-DNA adducts formation under simulated physiological conditions by CE-ICP-MS/MS.

The leading Pt(II)-based anticancer drugs have been used for decades; however, chemotherapy with their application is burdened with severe side effects. The administration of compounds capable of DNA platination in the form of prodrugs has the potential to overcome the drawbacks associated with their use. Progress toward their clinical application depends on establishing proper methodologies that would allow assessing their ability to bind to DNA in the biological environment. Herein, we propose implementing the approach based on the hyphenation of capillary electrophoresis with inductively coupled plasma tandem mass spectrometry (CE-ICP-MS/MS) for studying Pt-DNA adduct formation. The presented methodology opens the possibility to employ the multielement monitoring for studying the differences in the behavior of Pt(II) and Pt(IV) complexes and, interestingly, revealed the formation of various adducts with DNA and cytosol components for the latter one. Copyright © 2023 Elsevier B.V. All rights reserved.

Citation

Joanna Zajda, Paweł Borowiecki, Magdalena Matczuk. Effective monitoring of Platinum-DNA adducts formation under simulated physiological conditions by CE-ICP-MS/MS. Talanta. 2023 Nov 01;264:124749

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PMID: 37290334

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