Sperm head morphometry and chromatin alterations in bulls with different conception rates.

Animal reproduction science 2023 Aug

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The objective of this investigation was to analyze timed-AI conception rates (CRs) of different sires in light of their conventional semen quality parameters, sperm head morphometry, and chromatin alterations. Semen was collected in the field from six Angus bulls and used for the timed-AI of 890 suckled multiparous Nellore cows at a single farm. Semen batches were evaluated on the following in vitro parameters: sperm motility, concentration, and morphology, sperm head morphometry, and chromatin alteration types. The overall CR was 49% and Bulls 1 (43%) and 2 (40%) presented reduced (P < 0.05) pregnancies per AI compared to Bull 6 (61%), even though no differences were observed between their conventional semen quality parameters. Bull 1, however, presented higher (P = 0.0001) shape factor, smaller (P = 0.0025) antero-posterior symmetry, and elevated (P = 0.0141) Fourier 1 parameter, whereas Bull 2 exhibited a higher (P = 0.0023) percentage of chromatin alteration along the central axis of the sperm head. In conclusion, bulls with varying CRs may present sperm head morphometric differences and/or chromatin alterations while not presenting differences in conventional in vitro semen quality parameters. Although further studies are needed to elucidate the concrete implications of chromatin alterations on field fertility, sperm morphometric differences and chromatin alterations may be at least partially causative of the lower pregnancies per timed-AI of certain sires. Copyright © 2023 Elsevier B.V. All rights reserved.

Citation

José Andrés Nivia Riveros, Isabelle Cristina Pereira, Ana Carolina Bahia Teixeira, Juliana Horta Wilke Diniz, Alan Maia Borges, Juan Felipe García Díaz, Marcelo Emílio Beletti, Leticia Zoccolaro Oliveira. Sperm head morphometry and chromatin alterations in bulls with different conception rates. Animal reproduction science. 2023 Aug;255:107275