Detection of enrofloxacin residues in dairy products based on their fluorescence quenching effect on AgInS2 QDs.

Fengjiao Yang, Bingyu Yang, Xinyue Gu, Minghua Li, Kezhen Qi, Ya Yan

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2023 Nov 15

filter terms:

Water-soluble AgInS2 (AIS) quantum dots (QDs) were successfully prepared through the one-pot water phase method with thioglycolic acid (TGA) as the stabilizing agent. Because enrofloxacin (ENR) effectively quenches the fluorescence of AIS QDs, a highly-sensitive fluorescence detection method is proposed to detect ENR residues in milk. Under optimal detection conditions, there was a good linear relationship between the relative fluorescence quenching amount (ΔF/F0) of AgInS2 with ENR and ENR concentration (C). The detection range was 0.3125-20.00 μg/mL, r = 0.9964, and the detection limit (LOD) was 0.024 μg/mL (n = 11). The average recovery of ENR in milk ranged from 95.43 to 114.28%. The method established in this study has advantages including a high sensitivity, a low detection limit, simple operation and a low cost. The fluorescence quenching mechanism of AIS QDs with ENR was discussed and the dynamic quenching mechanism of light-induced electron transfer was proposed. Copyright © 2023 Elsevier B.V. All rights reserved.

Citation

Fengjiao Yang, Bingyu Yang, Xinyue Gu, Minghua Li, Kezhen Qi, Ya Yan. Detection of enrofloxacin residues in dairy products based on their fluorescence quenching effect on AgInS2 QDs. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 2023 Nov 15;301:122985