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A new high-pressure single-crystal diffraction setup has been designed and implemented at the Australian Synchrotron for collecting molecular and protein crystal structures. The setup incorporates a modified micro-Merrill-Bassett cell and holder designed specifically to fit onto the horizontal air-bearing goniometer, allowing high-pressure diffraction measurements to be collected with little to no modification of the beamline setup compared with ambient data collections. Compression data for the amino acid, L-threonine, and the protein, hen egg-white lysozyme, were collected, showcasing the capabilities of the setup. open access.


Stephanie A Boer, Jason R Price, Alan Riboldi-Tunnicliffe, Rachel Williamson, Robert Rostan, Aston Summers, Gemma F Turner, Isabelle Jones, Charles S Bond, Alice Vrielink, Andrew C Marshall, John Hitchings, Stephen A Moggach. High-pressure single-crystal diffraction at the Australian Synchrotron. Journal of synchrotron radiation. 2023 Jul 01;30(Pt 4):841-846

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PMID: 37318368

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