Generation of a homozygous RANGRF knockout hiPSC line by CRISPR/Cas9 system.

The RAN Guanine Nucleotide Release Factor (RANGRF) gene encodes the protein MOG1, which binds to Nav1.5 and facilitates its transport to the cell membrane. Nav1.5 mutations have been linked to various cardiac arrhythmias and cardiomyopathy. To investigate the role of RANGRF in this process, we utilized the CRISPR/Cas9 gene editing system to generate a homozygous RANGRF knockout hiPSC line. The availability of the cell line will prove to be an invaluable asset in the study of disease mechanisms and the testing of gene therapies for cardiomyopathy. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.

Citation

Meiling Jiang, Chengcheng Tang, Xian Luo, Xiaoqing Zhou, Min Chen, Yue Chi, Liangxue Lai, Qingjian Zou. Generation of a homozygous RANGRF knockout hiPSC line by CRISPR/Cas9 system. Stem cell research. 2023 Sep;71:103136

Mesh Tags

PMID: 37327621

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