Flow Cytometric Analysis and Sorting of Plant Chromosomes.

Petr Cápal, Mahmoud Said, István Molnár, Jaroslav Doležel

Methods in molecular biology (Clifton, N.J.) 2023

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chromosomes plant (1)

meristem (1)

plant chromosomes (2)

root tips (1)

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Flow cytometry offers a unique way of analyzing and manipulating plant chromosomes. During a rapid movement in a liquid stream, large populations can be classified in a short time according to their fluorescence and light scatter properties. Chromosomes whose optical properties differ from other chromosomes in a karyotype can be purified by flow sorting and used in a range of applications in cytogenetics, molecular biology, genomics, and proteomics. As the samples for flow cytometry must be liquid suspensions of single particles, intact chromosomes must be released from mitotic cells. This protocol describes a procedure for preparation of suspensions of mitotic metaphase chromosomes from meristem root tips and their flow cytometric analysis and sorting for various downstream applications. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Petr Cápal, Mahmoud Said, István Molnár, Jaroslav Doležel. Flow Cytometric Analysis and Sorting of Plant Chromosomes. Methods in molecular biology (Clifton, N.J.). 2023;2672:177-200