Strain dropouts reveal interactions that govern the metabolic output of the gut microbiome.

The gut microbiome is complex, raising questions about the role of individual strains in the community. Here, we address this question by constructing variants of a complex defined community in which we eliminate strains that occupy the bile acid 7α-dehydroxylation niche. Omitting Clostridium scindens (Cs) and Clostridium hylemonae (Ch) eliminates secondary bile acid production and reshapes the community in a highly specific manner: eight strains change in relative abundance by >100-fold. In single-strain dropout communities, Cs and Ch reach the same relative abundance and dehydroxylate bile acids to a similar extent. However, Clostridium sporogenes increases >1,000-fold in the ΔCs but not ΔCh dropout, reshaping the pool of microbiome-derived phenylalanine metabolites. Thus, strains that are functionally redundant within a niche can have widely varying impacts outside the niche, and a strain swap can ripple through the community in an unpredictable manner, resulting in a large impact on an unrelated community-level phenotype. Copyright © 2023 Elsevier Inc. All rights reserved.

Citation

Min Wang, Lucas J Osborn, Sunit Jain, Xiandong Meng, Allison Weakley, Jia Yan, William J Massey, Venkateshwari Varadharajan, Anthony Horak, Rakhee Banerjee, Daniela S Allende, E Ricky Chan, Adeline M Hajjar, Zeneng Wang, Alejandra Dimas, Aishan Zhao, Kazuki Nagashima, Alice G Cheng, Steven Higginbottom, Stanley L Hazen, J Mark Brown, Michael A Fischbach. Strain dropouts reveal interactions that govern the metabolic output of the gut microbiome. Cell. 2023 Jun 22;186(13):2839-2852.e21

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PMID: 37352836

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