How Membrane Contact Sites Shape the Phagophore.

During macroautophagy, phagophores establish multiple membrane contact sites (MCSs) with other organelles that are pivotal for proper phagophore assembly and growth. In S. cerevisiae, phagophore contacts have been observed with the vacuole, the ER, and lipid droplets. In situ imaging studies have greatly advanced our understanding of the structure and function of these sites. Here, we discuss how in situ structural methods like cryo-CLEM can give unprecedented insights into MCSs, and how they help to elucidate the structural arrangements of MCSs within cells. We further summarize the current knowledge of the contact sites in autophagy, focusing on autophagosome biogenesis in the model organism S. cerevisiae. © The Author(s) 2023.

Citation

Cristina Capitanio, Anna Bieber, Florian Wilfling. How Membrane Contact Sites Shape the Phagophore. Contact (Thousand Oaks (Ventura County, Calif.)). 2023 Jan-Dec;6:25152564231162495

PMID: 37366413

search → result