The diagnostic and prognostic value of a line immunoblot assay in Taiwanese patients with systemic sclerosis.

We aimed to evaluate the diagnostic performance and prognostic value of disease-specific antibodies and anti-Ro52 using a commercial line immunoblot assay (LIA) in Taiwanese patients with systemic sclerosis (SSc). We retrospectively enrolledall individuals at the Taichung Veterans General Hospital. We evaluated the diagnostic performance of LIA, anti-nuclear antibody (ANA) by indirect immunofluorescence (IIF) and also the association between the autoantibodies and the clinical phenotype using multivariable logistic regression. The LIA exhibited a sensitivity of 65.4% and a specificity of 65.4%, at the optimal cutoff values of 2 + signal intensity. By taking the result of ANA into consideration, the optimal cutoff point was redefined as 1+. We observed a higher risk of diffuse cutaneous SSc (dcSSc) in those with negative autoantibodies, positive anti-Scl-70, anti-RNA polymerase III, and anti-Ro-52. Interstitial lung disease (ILD) was associated with negative autoantibodies, as well as positive anti-Scl-70 and anti-Ro52. Anti-Ro52 positivity was also associated with pulmonary arterial hypertension (PAH) and gastrointestinal tract involvement. The presence of anti-Ro52 or the absence of SSc-specific autoantibodies may potentially indicate advanced diseases in patients with SSc. The incorporation of both IIF and LIA testing may improve the diagnostic specificity of SSc. Copyright © 2023 Elsevier B.V. All rights reserved.

Citation

Tsai-Hung Yen, Jun-Peng Chen, Tsu-Yi Hsieh, Wei-Ting Hung, Kuo-Lung Lai, Chia-Wei Hsieh, Hsin-Hua Chen, Wen-Nan Huang, Yi-Hsing Chen, Yi-Ming Chen. The diagnostic and prognostic value of a line immunoblot assay in Taiwanese patients with systemic sclerosis. Clinica chimica acta; international journal of clinical chemistry. 2023 Jul 01;547:117457

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PMID: 37390945

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