Exploring the molecular mechanism of linagliptin in osteosarcoma cell lines for anti-cancer activity.

Asiye Gok Yurttas, Mustafa Fatih Dasci

Pathology, research and practice 2023 Aug

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Finding new applications for widely used current drugs is a fast and effective technique for discovering new anticancer chemicals. Osteosarcoma (OS), the most prevalent form of bone cancer, has several side effects that significantly lower patients' quality of life. This study aims to systematically examine the anti-cancer activity of linagliptin (LG) in the osteosarcoma cell line Saos-2. MTT assays and flow cytometry were used to assess cell viability and apoptosis, respectively. qPCR array experiments were carried out to determine target gene expressions and explain the molecular mechanism of LG's action. Linagliptin treatment significantly decreased the viability of Saos-2 cells and hFOB1.19 cells (p < 0.001). The treatment also induced increased apoptotic effects in both Saos-2 cells (p < 0.001) and hFOB1.19 cells (p < 0.05). qPCR assays were conducted to assess cancer pathway analysis after applying specific quantities of LG to Saos-2 and hFOB1.19 cells. The findings of this study demonstrate that LG inhibits the proliferation of Saos-2 cells and induces cell death. LG supports cell death by suppressing the expression of specific genes involved in cancer pathways. Copyright © 2023 Elsevier GmbH. All rights reserved.

Citation

Asiye Gok Yurttas, Mustafa Fatih Dasci. Exploring the molecular mechanism of linagliptin in osteosarcoma cell lines for anti-cancer activity. Pathology, research and practice. 2023 Aug;248:154640