Luciano Aparecido de Almeida-Junior, Lisa Danielly Curcino Araujo, Giuliana Campos Chaves Lamarque, Maya Fernanda Manfrin Arnez, Yvonne Lorraine Kapila, Léa Assed Bezerra da Silva, Francisco Wanderley Garcia de Paula-Silva
Journal of endodontics 2023 OctTumor necrosis factor (TNF)-α is a pro-inflammatory cytokine that promotes biomineralization in vitro in dental pulp cells. However, the role of TNF-α-TNF receptor 1 (TNFR1) signaling in reparative dentin formation and related inflammatory pathways is not known. Therefore, the aim of this study was to evaluate the role of the TNF-α-TNFR1 axis in dental pulp repair following pulp capping in vivo. Dental pulp repair response of genetically deficient TNF-α receptor-1 mice (TNFR1-/-; n = 20) was compared with that of C57Bl6 mice (wild type [WT]; n = 20). Pulp capping was performed with mineral trioxide aggregate on the mandibular first molars of mice. After 7 and 70 days, tissues were collected and stained with hematoxylin and eosin for histopathological and histometric evaluation, and assessed by the Brown and Brenn methods for histomicrobiological analysis and by immunohistochemistry to localize TNF-α, Runt-related transcription factor 2, Dentin Sialoprotein (DSP) and Osteopontin (OPN) expression. Compared with WT mice, TNFR1-/- mice showed significantly decreased reparative dentin formation with a lower mineralized tissue area (P < .0001). Unlike WT mice, TNFR1-/- mice also exhibited significant dental pulp necrosis, neutrophil recruitment, and apical periodontitis formation (P < .0001) without bacterial tissue invasion. TNFR1-/- animals further exhibited decreased TNF-α, DSP, and OPN expression (P < .0001), whereas Runt-related transcription factor 2 expression was unchanged (P > .05). The TNF-α-TNFR1 axis is involved in reparative dentin formation following dental pulp capping in vivo. Genetic ablation of TNFR1 modified the inflammatory process and inhibited the expression of the DSP and OPN mineralization proteins, which culminated in dental pulp necrosis and development of apical periodontitis. Copyright © 2023 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
Luciano Aparecido de Almeida-Junior, Lisa Danielly Curcino Araujo, Giuliana Campos Chaves Lamarque, Maya Fernanda Manfrin Arnez, Yvonne Lorraine Kapila, Léa Assed Bezerra da Silva, Francisco Wanderley Garcia de Paula-Silva. Reparative Dentin Formation Following Dental Pulp Capping is Mediated by TNFR1 In Vivo. Journal of endodontics. 2023 Oct;49(10):1329-1336
PMID: 37423584
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