Vincenza Gragnaniello, Daniela Gueraldi, Andrea Puma, Anna Commone, Christian Loro, Chiara Cazzorla, Johannes Häberle, Alberto B Burlina
Journal of pediatric endocrinology & metabolism : JPEM 2023 Sep 26Carbamoyl phosphate synthetase 1 (CPS1) deficiency is a severe urea cycle disorder. Patients can present with hyperammonemic coma in the first days of life. Treatment includes nitrogen scavengers, reduced protein intake and supplementation with L-arginine and/or L-citrulline. N-carbamoyl glutamate (NCG) has been hypothesized to stimulate the residual CPS1 function, although only few patients are reported. We report a patient with neonatal-onset CPS1 deficiency who received NCG in association with nitrogen scavenger and L-citrulline. The patient carried the novel variants CPS1-c.2447A>G p.(Gln816Arg) and CPS1-c.4489T>C p.(Tyr1497His). The latter is localized in the C-terminal allosteric domain of the protein, and is implicated in the binding of the natural activator N-acetyl-L-glutamate. NCG therapy was effective in controlling ammonia levels, allowing to increase the protein intake. Our data show that the response to NCG can be indicated based on the protein structure. We hypothesize that variants in the C-terminal domain may be responsive to NCG therapy. © 2023 Walter de Gruyter GmbH, Berlin/Boston.
Vincenza Gragnaniello, Daniela Gueraldi, Andrea Puma, Anna Commone, Christian Loro, Chiara Cazzorla, Johannes Häberle, Alberto B Burlina. Variant in the allosteric domain of CPS1 protein associated with effectiveness of N-carbamoyl glutamate therapy in neonatal onset CPS1 deficiency. Journal of pediatric endocrinology & metabolism : JPEM. 2023 Sep 26;36(9):873-878
PMID: 37427576
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