Assessment of stable carbon isotope 13С/12С ratio in phthalates from surface waters using HPLC-HRMS-TOF approach.

Anton Kuzmin, Tatyana Grigoryeva, Alexander Gorshkov

Environmental science and pollution research international 2023 Aug

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A method for estimating the ratio of stable carbon isotopes 13С/12С in the composition of phthalates from surface water at a trace concentration level is proposed. It is based on the concentration of hydrophobic components of water using an analytical reversed phase HPLC column followed by their gradient separation and detection of eluted phthalates using a high-resolution time-of-flight mass spectrometer (ESI-HRMS-TOF) in the form of molecular ions. The ratio of stable carbon isotopes 13С/12C in phthalates is calculated as a ratio of integrals under the monoisotopic [M+1+H]+ and [M+H]+ peaks. The Δ13C value is calculated relatively to the 13C/12C ratio in commercial DnBP and DEHP phthalates used as standards. The minimal concentration of DnBP and DEHP in water required for a reliable determination of Δ13C value is estimated by the level of ca. 0.2 μg L-1. The technique has been verified during the monitoring of priority phthalates in the waters of Lake Baikal. © 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Citation

Anton Kuzmin, Tatyana Grigoryeva, Alexander Gorshkov. Assessment of stable carbon isotope 13С/12С ratio in phthalates from surface waters using HPLC-HRMS-TOF approach. Environmental science and pollution research international. 2023 Aug;30(37):87734-87742