The bacterial cytochrome P450 (CYP) CYP125 enzymes can competitively oxidise sitosterol in the presence of cholesterol.

Daniel Z Doherty, Amna Ghith, Ava Ho, James J De Voss, Stephen G Bell

Chemical communications (Cambridge, England) 2023 Jul 27

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Cholesterol catabolism is an important survival mechanism for the pathogenic Mycobacterium tuberculosis. Various other mycobacteria degrade not only cholesterol but plant sterols such as sitosterol and campesterol. In this work we demonstrate that the cytochrome P450 (CYP) CYP125 enzyme family is capable of sitosterol and campesterol side-chain oxidation and activation in these bacteria. We also show that the CYP142 and CYP124 cholesterol hydroxylating enzyme families are significantly less active for sitosterol hydroxylation compared to CYP125 enzymes.

Citation

Daniel Z Doherty, Amna Ghith, Ava Ho, James J De Voss, Stephen G Bell. The bacterial cytochrome P450 (CYP) CYP125 enzymes can competitively oxidise sitosterol in the presence of cholesterol. Chemical communications (Cambridge, England). 2023 Jul 27;59(61):9392-9395