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Because of its adaptability to high-throughput approaches and a low operating cost, the yeast two-hybrid (Y2H) assay remains the most widely used one for high-throughput protein-protein interactions (PPI) mapping experiments. Here we provide a detailed protocol for a liquid culture-based high-throughput binary protein-protein Y2H screen pipeline of a pool of 50 proteins used as baits against a collection of ~12,000 Arabidopsis proteins encoded by sequence-verified open reading frames (ORFs). © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.


Benoît Castandet, Claire Lurin, Étienne Delannoy, Dario Monachello. High-Throughput Protein-Protein Interactions Screening Using Pool-Based Liquid Yeast Two-Hybrid Pipeline. Methods in molecular biology (Clifton, N.J.). 2023;2690:161-177

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PMID: 37450147

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