Time- and cost-effective production of untagged recombinant MVA by flow virometry and direct virus sorting

Recombinant MVAs (rMVAs) are widely used both in basic and clinical research. Our previously developed Red-to-Green Gene Swapping Method (RGGSM), a cytometry-based Cell-Sorting protocol, revolves around the transient expression of a green fluorescent cytoplasmic marker, to subsequently obtain purified untagged rMVA upon loss of that marker by site-specific recombination. The standard RGSSM is quite costly in terms of bench work, reagents, and Sorting Facility fees. Although faster than other methods to obtain recombinant MVAs, the standard RGSSM still is time-consuming, taking at least 25 days to yield the final product.

Citation

Boselli Daniela, Maddalena Panigada, Simona Di Terlizzi, Monica Romanò, Emanuele Canonico, Chiara Villa, Claudia Minici, Eelco van Anken, Elisa Soprana, Antonio G. Siccardi. Time- and cost-effective production of untagged recombinant MVA by flow virometry and direct virus sorting Journal of Translational Medicine. 2023 Jul 23;21

PMID: 37482614

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