Correlation Engine 2.0
Clear Search sequence regions

  • ALDH1A1 (2)
  • Atg5 (8)
  • cadmium (12)
  • germ cell (3)
  • humans (1)
  • impairs (1)
  • level proteins (1)
  • meiosis (1)
  • mice (4)
  • mice knockout (1)
  • retinoic acid (1)
  • semen (1)
  • sertoli cells (12)
  • sperm count (1)
  • spermatogenesis (7)
  • STRA8 (1)
  • SYCP3 (1)
  • testis (4)
  • WT1 (1)
  • Sizes of these terms reflect their relevance to your search.

    Cadmium (Cd), one of the most common contaminants in diet and drinking water, impairs testicular germ cell development and spermatogenesis. Autophagy is essential for maintaining Sertoli cell function and Sertoli-germ cell communication. However, the role of Sertoli cell autophagy in Cd-caused spermatogenesis disorder remains unclear. Here, the mice of autophagy-related gene 5 (Atg5) knockouts in Sertoli cells were used to investigate the effect of autophagy deficiency on Cd-impaired spermatogenesis and its underlying mechanisms. Results showed that Sertoli cell-specific knockout of Atg5 exacerbated Cd-reduced sperm count and MVH (a specific marker for testicular germ cells) level in mice. Additionally, Sertoli cell Atg5 deficiency reduced the number of spermatocytes and decreased the level of meiosis-related proteins (SYCP3 and STRA8) in Cd-treated mouse testes. Loss of Atg5 in Sertoli cell exacerbated Cd-reduced the level of retinoic acid (RA) and retinal dehydrogenase (ALDH1A1 and ALDH1A) in mouse testes. Meanwhile, we found that the level of transcription factor WT1 was significantly downregulated in Atg5-/- plus Cd-treated testes. Further experiments showed that Wt1 overexpression restored Cd-decreased the levels of ALDH1A1 in Sertoli cells. Collectively, the above data suggest that knockout of Atg5 in Sertoli cell enhances the susceptibility of Cd-impaired testicular spermatogenesis. These findings provide new insights into autophagy of Sertoli cell preventing environmental toxicants-impaired testicular spermatogenesis. Copyright © 2023 Elsevier Ltd. All rights reserved.


    Yong-Wei Xiong, Dai-Xin Li, Zheng-Jia Ling, Lu-Lu Tan, Yu-Feng Zhang, Jin Zhang, Hao Li, Wei Chang, Hua-Long Zhu, Jun Zhang, Lan Gao, De-Xiang Xu, Lan Yang, Hua Wang. Loss of Atg5 in Sertoli cells enhances the susceptibility of cadmium-impaired testicular spermatogenesis in mice. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2023 Sep;179:113967

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 37506864

    View Full Text