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High-fat diets (HFD) have recently become a public health concern. We hypothesize that HFD induces exosomes biogenesis in the lung tissue of rat model. Sixteen adult male Wistar rats were fed with HFD or a regular chow diet for 3 months. The histopathological changes in lung tissues were measured by hematoxylin and eosin (H&E) staining. Bronchoalveolar lavage (BAL) was performed to assay exosomes by acetylcholinesterase enzyme (AhCE) activity. Real-time PCR (qPCR) was used to evaluate Rab27-b, Alix, and IL-1β expression, while the immunohistochemical examination was performed for CD81 expression in lung tissues. In addition, expression of IL-1β was detected by ELISA. We found pathological alterations in the lung tissue of HFD animals. AhCE activity along with the expression level of Rab27-b, Alix, and IL-1β was increased in HFD animals (p < 0.05). Immunohistochemical staining showed that expression of CD81 was increased in lung tissues of HFD animals compared with the control group (p < 0.05). Hence, HFD induced exosomes biogenesis and histopathological changes with IL-1β expression in rats' lung tissues. © 2023. The Author(s), under exclusive licence to Springer Nature B.V.


Maryam Shoaran, Mohammad Javad Behmand, Reza Rahbarghazi, Reza Mosaddeghi-Heris, Mahdi Ahmadi, Jafar Rezaie. High-fat diet-induced biogenesis of pulmonary exosomes in an experimental rat model. Molecular biology reports. 2023 Sep;50(9):7589-7595

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PMID: 37528312

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