A CG@MXene nanocomposite-driven E-CRISPR biosensor for the rapid and sensitive detection of Salmonella Typhimurium in food.

Miaolin Duan, Bingyan Li, Yawen He, Yijie Zhao, Yana Liu, Bo Zou, Yi Liu, Juhong Chen, Ruitong Dai, Xingmin Li, Fei Jia

Talanta 2024 Jan 01

filter terms:

In this study, we developed a novel electrochemical biosensor based on CRISPR/Cas12a (E-CRISPR) for the rapid and sensitive detection of Salmonella Typhimurium (S. Typhimurium). The CRISPR/Cas12a system was applied to identify S. Typhimurium gene and induce signal changes in electrochemical measurement. The colloidal gold and MXene (CG@MXene) nanocomposites were synthesized and immobilized to improve the performance of the biosensor by decreasing the background noise. The formation process of CG@MXene was well characterized, and experiment conditions were fully optimized. Under the optimal conditions, the proposed E-CRISPR biosensor exhibited excellent sensitivity for S. Typhimurium, with a limit of detection (LOD) of 160 CFU/mL, and great specificity against other common foodborne pathogens. Furthermore, the feasibility of the E-CRISPR biosensor was evaluated by analyzing S. Typhimurium-spiked chicken samples, with a recovery rate ranging from 100.46% to 106.37%. In summary, this research proposed a novel E-CRISPR biosensor from a new perspective to detect S. Typhimurium which can be an alternative approach for bacterial detection in the food supply chain. Copyright © 2023. Published by Elsevier B.V.

Citation

Miaolin Duan, Bingyan Li, Yawen He, Yijie Zhao, Yana Liu, Bo Zou, Yi Liu, Juhong Chen, Ruitong Dai, Xingmin Li, Fei Jia. A CG@MXene nanocomposite-driven E-CRISPR biosensor for the rapid and sensitive detection of Salmonella Typhimurium in food. Talanta. 2024 Jan 01;266(Pt 1):125011