Nonselective Expression of Short-Wavelength Cone Opsin Improves Learning in Mice with Retinal Degeneration in a Visually Guided Task.

O S Idzhilova, D E Kolotova, G R Smirnova, A Abonakour, D A Dolgikh, L E Petrovskaya, M P Kirpichnikov, M A Ostrovsky, A Yu Malyshev

Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections 2023 Jun

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The study explored the potential of an animal opsin nonselectively expressed in various neuronal elements of the degenerative retina to restore the impaired visual function. A knockout murine model of inherited retinal dystrophy was used. Mice were injected intravitreally with either a virus carrying the gene of short-wavelength cone opsin associated with a reporter fluorescent protein or a control virus carrying the sequence of a modified fluorescent protein with enhanced membrane tropism. Viral transduction induced pronounced opsin expression in ganglion, bipolar, and horizontal retinal neurons. Behavioral testing included the visually guided task in the trapezoid Morris water maze and showed a partial recovery of the learning ability in the mice whose retinas had been transduced with cone opsin. © 2023. Pleiades Publishing, Ltd.

Citation

O S Idzhilova, D E Kolotova, G R Smirnova, A Abonakour, D A Dolgikh, L E Petrovskaya, M P Kirpichnikov, M A Ostrovsky, A Yu Malyshev. Nonselective Expression of Short-Wavelength Cone Opsin Improves Learning in Mice with Retinal Degeneration in a Visually Guided Task. Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections. 2023 Jun;510(1):167-171