Membrane localization of LGG-1/GABARAP is dispensable for autophagy in C. elegans.

Most of the functions of LC3/GABARAP in macroautophagy/autophagy are considered to depend on their association with the phagophore membrane through a conjugation to a lipid. Using site-directed mutagenesis, we inhibited the conjugation of LGG-1, the single homolog of GABARAP in C. elegans. Mutants that express only cytosolic forms revealed an essential role for the cleaved form of LGG-1 in autophagy but also in an autophagy-independent embryonic function.

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Romane Leboutet, Céline Largeau, Emmanuel Culetto, Christophe Lefebvre, Thorsten Hoppe, Renaud Legouis. Membrane localization of LGG-1/GABARAP is dispensable for autophagy in C. elegans. Autophagy. 2023 Dec;19(12):3254-3255

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PMID: 37612881

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