Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot.

Bo Wang, Ya-Hui Wang, Yuan-Jie Deng, Quan-Hong Yao, Ai-Sheng Xiong

BMC plant biology 2023 Aug 24

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Betalain is a natural pigment with important nutritional value and broad application prospects. Previously, we produced betanin biosynthesis transgenic carrots via expressing optimized genes CYP76AD1S, cDOPA5GTS and DODA1S. Betanin can accumulate throughout the whole transgenic carrots. But the effects of betanin accumulation on the metabolism of transgenic plants and whether it produces unexpected effects are still unclear. The accumulation of betanin in leaves can significantly improve its antioxidant capacity and induce a decrease of chlorophyll content. Transcriptome and metabolomics analysis showed that 14.0% of genes and 33.1% of metabolites were significantly different, and metabolic pathways related to photosynthesis and tyrosine metabolism were markedly altered. Combined analysis showed that phenylpropane biosynthesis pathway significantly enriched the differentially expressed genes and significantly altered metabolites. Results showed that the metabolic status was significantly altered between transgenic and non-transgenic carrots, especially the photosynthesis and tyrosine metabolism. The extra consumption of tyrosine and accumulation of betanin might be the leading causes. © 2023. BioMed Central Ltd., part of Springer Nature.

Citation

Bo Wang, Ya-Hui Wang, Yuan-Jie Deng, Quan-Hong Yao, Ai-Sheng Xiong. Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot. BMC plant biology. 2023 Aug 24;23(1):402