Comparative genomic analyses reveal the genetic basis of the yellow-seed trait in Brassica napus.

Yellow-seed trait is a desirable breeding characteristic of rapeseed (Brassica napus) that could greatly improve seed oil yield and quality. However, the underlying mechanisms controlling this phenotype in B. napus plants are difficult to discern because of their complexity. Here, we assemble high-quality genomes of yellow-seeded (GH06) and black-seeded (ZY821). Combining in-depth fine mapping of a quantitative trait locus (QTL) for seed color with other omics data reveal BnA09MYB47a, encoding an R2R3-MYB-type transcription factor, as the causal gene of a major QTL controlling the yellow-seed trait. Functional studies show that sequence variation of BnA09MYB47a underlies the functional divergence between the yellow- and black-seeded B. napus. The black-seed allele BnA09MYB47aZY821, but not the yellow-seed allele BnA09MYB47aGH06, promotes flavonoid biosynthesis by directly activating the expression of BnTT18. Our discovery suggests a possible approach to breeding B. napus for improved commercial value and facilitates flavonoid biosynthesis studies in Brassica crops. © 2023. Springer Nature Limited.

Citation

Cunmin Qu, Meichen Zhu, Ran Hu, Yongchao Niu, Si Chen, Huiyan Zhao, Chengxiang Li, Zhen Wang, Nengwen Yin, Fujun Sun, Zhiyou Chen, Shulin Shen, Guoxia Shang, Yan Zhou, Xingying Yan, Lijuan Wei, Liezhao Liu, Bin Yi, Jinmin Lian, Jiang Li, Zhanglin Tang, Ying Liang, Xinfu Xu, Rui Wang, Jiaming Yin, Huafang Wan, Hai Du, Wei Qian, Yourong Chai, Qingyuan Zhou, Yajun He, Silin Zhong, Xiao Qiu, Hao Yu, Hon-Ming Lam, Kun Lu, Fuyou Fu, Jiana Li. Comparative genomic analyses reveal the genetic basis of the yellow-seed trait in Brassica napus. Nature communications. 2023 Aug 25;14(1):5194

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PMID: 37626056

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