Ultrasensitive digital immunoassays for SOD1 conformation in amyotrophic lateral sclerosis.

Lisa Morichon, Christophe Hirtz, Laurent Tiers, Alexandre Mezghrani, Cédric Raoul, Florence Esselin, Elisa De La Cruz, Jean-Pierre Julien, William Camu, Sylvain Lehmann

Bioanalysis 2023 Aug

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Aim: The aim of this study was to detect misfolded Cu/Zn SOD1 as a potential biomarker for amyotrophic lateral sclerosis (ALS). Materials & methods: Two ultrasensitive immunodetection assays were developed for the quantification of total and misfolded SOD1. Results: The detection of total and misfolded SOD1 was possible in human serum and cerebrospinal fluid. Total SOD1 was increased in cerebrospinal fluid from ALS patients. Misfolded SOD1 had low and variable expression in both control and ALS patient samples. Conclusion: These assays hold promise for improving our understanding of ALS and its detection, and could lead to more effective treatment options in the future. Further studies in larger cohorts are now required.

Citation

Lisa Morichon, Christophe Hirtz, Laurent Tiers, Alexandre Mezghrani, Cédric Raoul, Florence Esselin, Elisa De La Cruz, Jean-Pierre Julien, William Camu, Sylvain Lehmann. Ultrasensitive digital immunoassays for SOD1 conformation in amyotrophic lateral sclerosis. Bioanalysis. 2023 Aug;15(15):927-936