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Tieguanyin (TGY) is one of top ten famous teas in China, but in the process of brand building there is the phenomenon of falsehood, thus harming the interests of consumers. To solve theadulterate problem of TGY, a colorimetric sensor array (CSA) based onperoxidase activity of nanozyme was constructed. Nanozymes can catalyze 3,3',5,5'-tetramethylbenzidine (TMB) to 3,3',5,5'-tetramethyl -[1,1'-bis(cyclohexyl)]-2,2',5,5'-tetraene-4,4'-diimine (oxTMB), while the tea polyphenols (TPs) can inhibit this process, and the degree of inhibition varies significantly with the reaction time. We selected two nanozymesand three reaction time points to construct CSA. It can successfully distinguish TPsin TGY. The discriminative analysiscan achieve: (1)distinction between TGY and adulterated tea, (2)discrimination of TGY in various seasons and seasonal adulteration in different degrees. The method constructed in this work is promising for both the class and quality differentiation of TGY and other teas with TPs as the main activity. Copyright © 2023 Elsevier Ltd. All rights reserved.


Xiaoyu Yang, Zhichun Bi, Chenghui Yin, Shuyi Zhang, Donghui Song, Hui Huang, Yongxin Li. A colorimetric sensor array based on peroxidase activity nanozyme for the highly efficient differential sensing of tea polyphenols and Tieguanyin adulteration. Food chemistry. 2024 Jan 30;432:137265

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PMID: 37657343

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