Programming an Enhanced Uptake and the Intracellular Fate of Magnetic Microbeads.

This study compares two kinds of magnetic microbeads with different surface features and cell entry pathways, aiming to provide insights into how to program their cell uptake and intracellular fate. It is found that a rougher surface enhances the cell uptake of the microbeads, regardless of whether they are pulled by a magnetic field gradient or adsorbed by the cell membrane. However, the entry route affects the intracellular localization of the microbeads: The magnetically dragged microbeads reach the cytoplasm, while the adsorbed microbeads stay in the late endosomes and lysosomes. This suggests that different strategies can be used to target different cellular compartments with magnetic microbeads. Moreover, it is demonstrated that the cells containing the microbeads can be moved and regrown at specific locations by applying a magnetic field gradient, showing the potential of these magnetic microbeads for cell delivery and manipulation. © 2023 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.

Citation

Ecem Tiryaki, Saida Ortolano, Gustavo Bodelón, Verónica Salgueiriño. Programming an Enhanced Uptake and the Intracellular Fate of Magnetic Microbeads. Advanced healthcare materials. 2023 Dec;12(30):e2301415

Mesh Tags

PMID: 37660272

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