HDAC3 improves intestinal function of mice by regulating cGAS-Sting pathway of intestinal glial cells.

Pu Li, Zhaohui Zheng, Jing Qi, Yanyao Gao, Liu Yang, Lu Li, Changjun Gao

Molecular immunology 2023 Oct

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It is found that HDAC3 may be a potential therapeutic target for intestinal related diseases. At present, the role and mechanism of HDAC3 in the pathogenesis of severe acute pancreatitis (SAP) have not been reported, which needs to be further explored. The SAP mouse model was established and the expression of HDAC3 was detected by immunohistochemistry. H&E staining showed the intestinal pathological state of SAP mice. The expression of HDAC3 was measured by real-time quantitative PCR (RT qPCR) and Western blot. Apoptosis kit was used to determine cell apoptosis rate. The level of inflammatory factors was detected by ELISA kits. The expressions of HDAC3, cGAS and Sting were significantly increased in SAP patients and SAP mice. Silencing HDAC3 promoted the proliferation and adhesion of intestinal glial cells and inhibited the inflammation and apoptosis of intestinal epithelial cells. In addition, silencing HDAC3 inhibited oxidative stress in intestinal epithelial cells. Furthermore, silencing HDAC3 inhibited the activation of cGAS-Sting pathway in intestinal glial cells. More importantly, silencing HDAC3 alleviates intestinal barrier function in SAP mice. HDAC3 inhibition improves acute pancreatitis in mice by regulating cGAS-Sting pathway of intestinal glial cells. Copyright © 2023 Elsevier Ltd. All rights reserved.

Citation

Pu Li, Zhaohui Zheng, Jing Qi, Yanyao Gao, Liu Yang, Lu Li, Changjun Gao. HDAC3 improves intestinal function of mice by regulating cGAS-Sting pathway of intestinal glial cells. Molecular immunology. 2023 Oct;162:95-101